Protein Phosphatase 2A
Background
Purchase
- DSHB CPTC-PP2A-1
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-PP2A-1 Western Blot
Western Blot using CPTC-PP2A-1 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-PP2A-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-PP2A-1 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-PP2A-1 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-PP2A-1 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
SPR
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CPTC-PP2A-1 SPR
Kinetic titration data for PP2A-1 Ab (622A1.1) using Biacore SPR method
Characterization SOP Files
Background
Purchase
- DSHB CPTC-PP2A-2
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-PP2A-2 Western Blot
Western Blot using CPTC-PP2A-2 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-PP2A-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
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CPTC-PP2A-2 SPR
Kinetic titration data for PP2A-2 Ab (622A3.1) using Biacore SPR method
Characterization SOP Files
Background
Purchase
- DSHB CPTC-PP2A-3
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-PP2A-3 Western Blot
Western Blot using CPTC-PP2A-3 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-PP2A-3 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
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CPTC-PP2A-3 Protein Array
Protein Array in which CPTC-PP2A-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
Purchase
- DSHB CPTC-PP2A-4
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-PP2A-4 Western Blot
Western Blot using CPTC-PP2A-4 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-PP2A-4 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Background
Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than
the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen
LTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRT
WYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAF
LCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQF
LPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHS
NQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG
LNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTW
YAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAF
LCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQ
FLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAE
HSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG
Links
- Uniprot - Uniprot PP2A
- EnsEMBL - EnsEMBL PP2A
- Entrez - Entrez PP2A
Characterization Data
Gel
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CPTC-PP2A (Ag 10501) PAGE
PAGE of Ag 10501 with molecular weight standards in lane 1
SOPs
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