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Proliferating Cell Nuclear Antigen Peptide 1

Antibody: CPTC-PCNA-1

Background

Catalog Number:
CPTC-PCNA-1
Target Antigen:
Proliferating Cell Nuclear Antigen Peptide 1
Isotype:
IgG
Species:
Rabbit
Last Updated:
05/22/2014
Antigen Recognition(s):
peptide

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Characterization Data [Compare Characterization Data]

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-PCNA-1 Indirect ELISA (peptide)

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

Antigen: Proliferating Cell Nuclear Antigen Peptide 1

Background

NCI Identification Number:
00035
Antigen Name:
Proliferating Cell Nuclear Antigen Peptide 1
CPTC Name:
CPTC-PCNA Peptide 1
Keywords:
Cyclin; DNA Polymerase Delta Auxiliary Protein
Function:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Chromosomal Localization:
20pter-p12
Expression System:
NCBI Accession Number:
NP_002583.1
Swiss Prot Accession Number:
P12004
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LVQGSILK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
880
Last Updated:
06/11/2013

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Characterization Data

SOPs

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