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Ornithine Decarboxylase 1

Antibody: CPTC-ODC1-1

Background

Catalog Number:
CPTC-ODC1-1
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG1
Species:
Mouse
Last Updated:
02/25/2013
Antigen Recognition(s):
recombinant full-length

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-ODC1-1 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-ODC1-1 Western Blot

Western Blot Using CPTC-ODC1-1 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-ODC1-1
Click to enlarge

CPTC-ODC1-1 Indirect ELISA

Indirect ELISA of CPTC-ODC1-1

Characterization SOP Files

NCI 60 Protein Array

CPTC-ODC1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-ODC1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-ODC1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

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Antibody: CPTC-ODC1-2

Background

Catalog Number:
CPTC-ODC1-2
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/24/2012
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-ODC1-2 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-ODC1-2 Western Blot

Western Blot Using CPTC-ODC1-2 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-ODC1-2
Click to enlarge

CPTC-ODC1-2 Indirect ELISA

Indirect ELISA of CPTC-ODC1-2

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-ODC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-ODC1-2 NCI60 Protein Array

Protein Array in which CPTC-ODC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

Click to enlarge image Immuno-histochemistry of CPTC-ODC1-2 for NCI60 Cell Line Array at titer 1:500 0=NEGATIVE 1=WEAK (red) 2=MODERATE(blue) 3=STRONG(green) Titer: 1:100
Click to enlarge

CPTC-ODC1-2 IHC NCI60

Immuno-histochemistry of CPTC-ODC1-2 for NCI60 Cell Line Array at titer 1:500
0=NEGATIVE
1=WEAK (red)
2=MODERATE(blue)
3=STRONG(green)
Titer: 1:100

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-ODC1-2. Titer: 1:100
Click to enlarge

CPTC-ODC1-2 IHC Tissue

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-ODC1-2. Titer: 1:100

Characterization SOP Files

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Antibody: CPTC-ODC1-3

Background

Catalog Number:
CPTC-ODC1-3
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/25/2013
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-ODC1-3 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-ODC1-3 Western Blot

Western Blot Using CPTC-ODC1-3 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-ODC1-3
Click to enlarge

CPTC-ODC1-3 Indirect ELISA

Indirect ELISA of CPTC-ODC1-3

Characterization SOP Files

NCI 60 Protein Array

CPTC-ODC1-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-ODC1-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-ODC1-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for ODC1-3 Ab (615A3.1) using Biacore SPR method
Click to enlarge

CPTC-ODC1-3 SPR

Kinetic titration data for ODC1-3 Ab (615A3.1) using Biacore SPR method

Characterization SOP Files

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Antigen: Ornithine Decarboxylase 1

Background

NCI Identification Number:
10532
Antigen Name:
Ornithine Decarboxylase 1
CPTC Name:
CPTC-ODC1
Keywords:
ODC1; EC 4.1.1.17; ODC
Function:
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.
Chromosomal Localization:
2p25
Expression System:
E. Coli
NCBI Accession Number:
BC025296
Swiss Prot Accession Number:
P11926
DNA Source:
HIP:HsCD00005772
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
53205
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDIL
KKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSL
GVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVL
RIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDP
ETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINP
ALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDES
SEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCD
GLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPA
WQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Native Sequence:
MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKH
LRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSLGVP
PERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIA
TDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETF
VQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALD
KYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQ
TFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLD
RIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQL
MQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Calculated Isoelectric Point:
5.1
Molecular Weight:
51420
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Kinetic titration data for ODC1-3 Ab (615A3.1) using Biacore SPR method
Click to enlarge

Ag 10532 Gel

PAGE of Ag 10532 with molecular weight standards

SOPs

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