Nucleoside Diphosphate Kinase B
Background
Purchase
- DSHB CPTC-NME2-1
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-NME2-1 Western Blot
Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-NME2-1 ELISA
Indirect ELISA of CPTC-NME2-1
Characterization SOP Files
Cross Reactivity Data
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Cross Reactivity Studies
This table shows the cross reactivity between NME1 and NME2.
Characterization SOP Files
SPR
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CPTC-NME2-1 SPR
Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method
Characterization SOP Files
Immuno-MS
Download NME2-1.pdf (123.0 KB)CPTC-NME2-1 Immuno-MS
This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.
Background
Purchase
- DSHB CPTC-NME2-2
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-NME2-2 Western Blot
Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-NME2-2 ELISA
Indirect ELISA of CPTC-NME2-2
Characterization SOP Files
Cross Reactivity Data
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Cross Reactivity Studies
This table shows the cross reactivity between NME1 and NME2.
Characterization SOP Files
NCI 60 Protein Array
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CPTC-NME2-2 Protein Array
Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
IHC NCI60
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CPTC-NME2-2 IHC NCI60
Immunohistochemistry of CPTC-NME2-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50
Characterization SOP Files
SPR
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CPTC-NME2-2 SPR
Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method
Characterization SOP Files
Immuno-MS
Download NME2-2.pdf (118.8 KB)CPTC-NME2-2 Immuno-MS
This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry.
Background
Purchase
- DSHB CPTC-NME2-3
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-NME2-3 Western Blot
Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-NME2-3 Indirect ELISA
Indirect ELISA of CPTC-NME2-3
Characterization SOP Files
Cross Reactivity Data
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Cross Reactivity Studies
This table shows the cross reactivity between NME1 and NME2.
Characterization SOP Files
SPR
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CPTC-NME2-3 SPR
Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method
Characterization SOP Files
Immuno-MS
Download NME2-3.pdf (125.8 KB)CPTC-NME2-3 Immuno-MS
This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.
Background
Acts as a transcriptional activator of the c-Myc gene; binds DNA nonspecifically
EQKGFRLVAMKFLRASEEHLKQHYIDLKDRP
FFPGLVKYMNSGPVVAMVWEGLNVVKTGRVM
LGETNPADSKPGTIRGDFCIQVGRNIIHGSD
SVKSAEKEISLWFKPEELVDYKSCAHDWVYE
GFRLVAMKFLRASEEHLKQHYIDLKDRPFFP
GLVKYMNSGPVVAMVWEGLNVVKTGRVMLGE
TNPADSKPGTIRGDFCIQVGRNIIHGSDSVK
SAEKEISLWFKPEELVDYKSCAHDWVYE
Links
- Uniprot - Uniprot NME2
- EnsEMBL - EnsEMBL NME2
Characterization Data
Gel
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PAGE of ANL antigen 10385
Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.
SOPs
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