Skip to Content

Clinical Proteomics Technologies for Cancer

  1. Contact Us
  2. CPTAC Home
  3. CPTAC Intranet
  4. Sign Up for Email Updates

Nucleoside Diphosphate Kinase A (nm23-H1)

Antibody: CPTC-NME1-1

Background

Catalog Number:
CPTC-NME1-1
Target Antigen:
Nucleoside Diphosphate Kinase A (nm23-H1)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-NME1-1 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-NME1-1 Western Blot

Western Blot using CPTC-NME1-1 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-NME1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows the cross reactivity between NME1 and NME2
Click to enlarge

Cross Reactivity Studies

This table shows the cross reactivity between NME1 and NME2

Characterization SOP Files

NCI 60 Protein Array

CPTC-NME1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-NME1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-NME1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

[top]
Antibody: CPTC-NME1-2

Background

Catalog Number:
CPTC-NME1-2
Target Antigen:
Nucleoside Diphosphate Kinase A (nm23-H1)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/28/2013
Antigen Recognition(s):
recombinant full-length

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-NME1-2 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-NME1-2 Western Blot

Western Blot using CPTC-NME1-2 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-NME1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows the cross reactivity data for NME1 and NME2.
Click to enlarge

Cross Reactivity Studies

This table shows the cross reactivity data for NME1 and NME2.

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-NME1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-NME1-2 NCI 60 Protein Array

Protein Array in which CPTC-NME1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

Click to enlarge image Immunohistochemistry of CPTC-NME1-2 for NCI60 Cell Line Array. Data scored as: 0=NEGATIVE 1=WEAK (red) 2=MODERATE (blue) 3=STRONG (green) Titer: 1:50
Click to enlarge

CPTC-NME1-2 IHC NCI60

Immunohistochemistry of CPTC-NME1-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-NME1-2. Titer: 1:50
Click to enlarge

CPTC-NME1-2 IHC Tissue

Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-NME1-2. Titer: 1:50

Characterization SOP Files

[top]
Antibody: CPTC-NME1-3

Background

Catalog Number:
CPTC-NME1-3
Target Antigen:
Nucleoside Diphosphate Kinase A (nm23-H1)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-NME1-3 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-NME1-3 Western Blot

Western Blot using CPTC-NME1-3 as primary Ab against NME1 (Ag 10263) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-NME1-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows the cross reactivity between NME1 and NME2.
Click to enlarge

Cross Reactivity Studies

This table shows the cross reactivity between NME1 and NME2.

Characterization SOP Files

NCI 60 Protein Array

CPTC-NME1-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-NME1-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-NME1-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

[top]
Antibody: CPTC-NME1-4

Background

Catalog Number:
CPTC-NME1-4
Target Antigen:
Nucleoside Diphosphate Kinase A (nm23-H1)
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-NME1-4 as primary Ab against NME1 (rAg 10263) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-NME1-4 Western blot

Western Blot using CPTC-NME1-4 as primary Ab against NME1 (rAg 10263) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-NME1-4 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

NCI 60 Protein Array

CPTC-NME1-4 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-NME1-4 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-NME1-4 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

[top]
Antibody: CPTC-NME1-5

Background

Catalog Number:
CPTC-NME1-5
Target Antigen:
Nucleoside Diphosphate Kinase A (nm23-H1)
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-NME1-5 as primary Ab against NME1 (rAg 10263) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-NME1-5 Western blot

Western Blot using CPTC-NME1-5 as primary Ab against NME1 (rAg 10263) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-NME1-5 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

NCI 60 Protein Array

CPTC-NME1-5 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-NME1-5 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-NME1-5 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

[top]
Antigen: Nucleoside Diphosphate Kinase A (nm23-H1)

Background

NCI Identification Number:
10263
Antigen Name:
Nucleoside Diphosphate Kinase A (nm23-H1)
CPTC Name:
CPTC-NME1
Keywords:
NME1; AWD; GAAD; NDPK-A; NDPKA; NM23; NM23-H1; nm23-H1; EC 2.7.4.6; OTTHUMP00000174772
Function:
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A'(encoded by this gene) and 'B'(encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Major role in the synthesis of nucleoside triphosphates other than ATP.
Chromosomal Localization:
17q21.33
Expression System:
E. Coli
NCBI Accession Number:
BC000293
Swiss Prot Accession Number:
P15531
DNA Source:
HIP : HsCD00001887
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
22523
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMANCERTFIAIKPDGVQRGLVGEIIKRF
EQKGFRLVGLKFMQASEDLLKEHYVDLKDRP
FFAGLVKYMHSGPVVAMVWEGLNVVKTGRVM
LGETNPADSKPGTIRGDFCIQVGRNIIHGSD
SVESAEKEIGLWFHPEELVDYTSCAQNWIYE
Native Sequence:
MANCERTFIAIKPDGVQRGLVGEIIKRFEQK
GFRLVGLKFMQASEDLLKEHYVDLKDRPFFA
GLVKYMHSGPVVAMVWEGLNVVKTGRVMLGE
TNPADSKPGTIRGDFCIQVGRNIIHGSDSVE
SAEKEIGLWFHPEELVDYTSCAQNWIYE
Calculated Isoelectric Point:
5.83
Molecular Weight:
17421
Last Updated:
11/14/2012

Links

Characterization Data

SOPs

[top]

Adobe Reader™ required.

Get Adobe Reader for free at Adobe.com