Methyl CpG Binding Protein 1
Background
Purchase
- DSHB CPTC-MBD1-1
External Links
- Human Protein Atlas CPTC-MBD1-1
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-MBD1-1 Western Blot
Western Blot using CPTC-MBD1-1 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-MBD1-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-MBD1-1 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-MBD1-1 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-MBD1-1 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Background
Purchase
- DSHB CPTC-MBD1-2
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-MBD1-2 Western Blot
Western Blot using CPTC-MBD1-2 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-MBD1-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-MBD1-2 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-MBD1-2 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-MBD1-2 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Background
Purchase
- DSHB CPTC-MBD1-3
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-MBD1-3 Western Blot
Western Blot using CPTC-MBD1-3 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-MBD1-3 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
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CPTC-MBD1-3 Protein Array
Protein Array in which CPTC-MBD1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of
these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five
transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from
methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor
and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as
the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1
and isoform 2 can also repress transcription from unmethylated promoters.
KRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSI
GSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFAS
ALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVE
RLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEAS
DTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMD
DSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVS
QSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQ
RSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ
VTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIG
SDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFAS
ALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANV
ERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSE
ASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIA
VMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLT
REVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQ
RSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ
Links
- EnsEMBL - EnsEMBL MBD1
- Entrez - Entrez MBD1
- Uniprot - Uniprot MBD1
Characterization Data
SOPs
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