KIAA1967


Background

Catalog Number:

CPTC-KIAA1967-1

RRID:

AB_2617284

Target Antigen:

KIAA1967

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (164.1 KB)

CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-KIAA1967-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-KIAA1967-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-KIAA1967-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-KIAA1967-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-KIAA1967-1 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-KIAA1967-1 Western blot

Result: Positive

Western Blot using CPTC-KIAA1967-1 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-KIAA1967-2

RRID:

AB_2617285

Target Antigen:

KIAA1967

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear bodies. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-KIAA1967-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear bodies. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-KIAA1967-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  NCI 60 Protein Array

CPTC-KIAA1967-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-KIAA1967-2 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-KIAA1967-2 Western blot

Result: Positive

Western Blot using CPTC-KIAA1967-2 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

NCI Identification Number:

00056

Antigen Name:

KIAA1967

CPTC Name:

CPTC-KIAA1967

Aliases:

DBC1; DBC-1; NET35; P30 DBC; DBIRD Complex Subunit KIAA1967; Deleted In Breast Cancer 1; P30 DBC Protein; P30DBC; DBC.1; Deleted In Breast Cancer Gene 1 Protein

Function:

Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity

Chromosomal Localization:

8p22

Expression System:

E. coli

Accession Number:

NP_066997.3

UniProt Accession Number:

Q8N163

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS pH 7.4

Expressed Sequence:

MHHHHHHSSGRENLYFQGKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYT
VDSPICDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLSQPFSLHHPSRIQ
VSSEKEAAPDAGAEPITADSDPAYSSKVLLLSSPGLEELYRCCMLFVDDM
AEPRETPEHPLKQIKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVR
TAIRCAQAQTGIDLSGCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTI
MPTLEEWEALCQQKAAEAAPPTQEAQGETEPTEQAPDALEQAADTSRRNA
ETPEATTQQETDTDLPEAPPPPLEPAVIARPGCVNLSLHGIVEDRRPKER
ISFEVMVLAELFLEMLQRDFGYRVYKMLLSLPEKVVSPPEPEKEEAAKEE
ATKEEEAIKEEVVKEPKDEAQNEGPATES

Native Sequence:

Calculated Isoelectric Point:

4.7

Molecular Weight:

48265

Last Updated:

10/23/2013

Links

Characterization Data

Gel

Click to enlarge image PAGE of KIAA1967 (rAg 00056) with molecular weight standards in lane 1
Click image to enlarge

CPTC-KIAA1967 PAGE

PAGE of KIAA1967 (rAg 00056) with molecular weight standards in lane 1

SOPs

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