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Indoleamine-pyrrole 2,3 dioxygenase

Antibody: CPTC-IDO1-1

Background

Catalog Number:
CPTC-IDO1-1
Target Antigen:
Indoleamine-pyrrole 2,3 dioxygenase
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-IDO1-1 Western Blot

Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-IDO1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

NCI 60 Protein Array

CPTC-IDO1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-IDO1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-IDO1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

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Antibody: CPTC-IDO1-2

Background

Catalog Number:
CPTC-IDO1-2
Target Antigen:
Indoleamine-pyrrole 2,3 dioxygenase
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):
recombinant full-length

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-IDO1-2 Western Blot

Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-IDO1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

NCI 60 Protein Array

CPTC-IDO1-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-IDO1-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-IDO1-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

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Antigen: Indoleamine-pyrrole 2,3 dioxygenase

Background

NCI Identification Number:
10898
Antigen Name:
Indoleamine-pyrrole 2,3 dioxygenase
CPTC Name:
CPTC-IDO1
Keywords:
CD107B; EC 1.13.11.52; IDO; INDO
Function:
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
Chromosomal Localization:
8p12 - p11
Expression System:
E. Coli
NCBI Accession Number:
BC027882.1
Swiss Prot Accession Number:
P14902
DNA Source:
OB : MHS1010-7430088
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
51130
Buffers:
PBS
Expressed Sequence:
SNAMAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIA
KHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMA
YVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWK
KKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVI
PTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFF
SVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCF
DVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREF
VLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKEN
KTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG
Native Sequence:
MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKH
LPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAY
VWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWK
KKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKV
IPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKA
FFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVF
QCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPS
VREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQ
QPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG
Calculated Isoelectric Point:
6.87
Molecular Weight:
45.33
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-IDO1 PAGE

PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1

SOPs

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