Catalog Number:
CPTC-Gelsolin-1
RRID:
AB_2263829
Target Antigen:
Gelsolin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-Gelsolin-1 CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Result: Positive
Western Blot using CPTC-Gelsolin-1 as primary Ab against Ag 10594 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-Gelsolin-2
RRID:
AB_2617260
Target Antigen:
Gelsolin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Result: Positive
This table summarizes the results of the pairing experiment with Gelsolin antibodies.
Result: Positive
Kinetic titration data for Gelsolin-2 using Biacore SPR method
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-Gelsolin-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).
Result: Positive
Western Blot using CPTC-Gelsolin-2 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-Gelsolin-3
RRID:
AB_2617261
Target Antigen:
Gelsolin
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Result: Positive
This table summarizes the results of the pairing experiment with Gelsolin antibodies.
Result: Positive
Kinetic titration data for Gelsolin-3 antibody using Biacore SPR method
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-Gelsolin-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).
Result: Positive
Western Blot using CPTC-Gelsolin-3 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-Gelsolin-4
RRID:
AB_2617262
Target Antigen:
Gelsolin
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Result: Positive
This table summarizes the results of the pairing experiment with Gelsolin antibodies.
Result: Positive
Kinetic titration data for Gelsolin-4 antibody using Biacore SPR method
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-Gelsolin-4 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).
Result: Positive
Western Blot using CPTC-Gelsolin-4 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10594
Antigen Name:
Gelsolin
CPTC Name:
CPTC-Gelsolin
Aliases:
amyloidosis, Finnish type; GSN; ADF; AGEL; Brevin; DKFZp313L0718; OTTHUMP00000022011; OTTHUMP00000162202
Function:
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments
to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
Chromosomal Localization:
9q33
Expression System:
E. Coli
Accession Number:
BC026033
UniProt Accession Number:
P06396
DNA Source:
HIP:HsCD00005767
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
115405
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAATASRGASQAGAPQGRVPEARPNSMVVEHPEFLKAGKEPGLQIWRVE
KFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDE
SGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVAS
GFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNI
HQWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVL
GPKPALPAGTEDTAKEDAANRKLAKLYKVSNGAGTMSVSLVADENPFAQG
ALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQ
TQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDA
ATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSY
IILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQS
RVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAG
ATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRV
LRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSN
KIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEAL
TSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLD
RAMAELAA
Native Sequence:
MAPHRPAPALLCALSLALCALSLPVRAATASRGASQAGAPQGRVPEARPN
SMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKT
VQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQ
GFESATFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRA
TEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKATQVSKGIRDN
ERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKL
YKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQA
NTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPD
QTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIW
RIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQ
DEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGG
TSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPS
AAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAA
YRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVML
LDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQG
FEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA
Calculated Isoelectric Point:
5.71
Molecular Weight:
83231
Last Updated:
09/01/2008
PAGE of Ag 10594 with molecular weight standards in lane 1
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