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Lactoylglutathione lyase

Antibody: CPTC-GLO1-1

Background

Catalog Number:
CPTC-GLO1-1
Target Antigen:
Lactoylglutathione lyase
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-GLO1-1 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-GLO1-1 Western Blot

Western Blot using CPTC-GLO1-1 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Cell Lysate Western Blot

Click to enlarge image Western Blot using CPTC-GLO1-1 as primary Ab against cell lysate from NCI-H522 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-GLO1-1 Cell Lysate Western blot

Western Blot using CPTC-GLO1-1 as primary Ab against cell lysate from NCI-H522 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-GLO1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-GLO1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-GLO1-1 NCI 60 Protein Array

Protein Array in which CPTC-GLO1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

Click to enlarge image Immuno-histochemistry of CPTC-GLO1-1 for NCI60 Cell Line Array at titer Titer: 1:50 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green)
Click to enlarge

CPTC-GLO1-1 IHC NCI60

Immuno-histochemistry of CPTC-GLO1-1 for NCI60 Cell Line Array at titer Titer: 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for GLO1-1 Ab (615A3.1) using Biacore SPR method
Click to enlarge

CPTC-GLO1-1 SPR

Kinetic titration data for GLO1-1 Ab (615A3.1) using Biacore SPR method

Characterization SOP Files

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Antibody: CPTC-GLO1-2

Background

Catalog Number:
CPTC-GLO1-2
Target Antigen:
Lactoylglutathione lyase
Isotype:
IgG2b
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-GLO1-2 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-GLO1-2 Western Blot

Western Blot using CPTC-GLO1-2 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-GLO1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-GLO1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-GLO1-2 NCI60 Protein Array

Protein Array in which CPTC-GLO1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

Click to enlarge image Immunohistochemistry of CPTC-GLO1-2 for NCI60 Cell Line Array. Data scored as: 0=NEGATIVE 1=WEAK (red) 2=MODERATE (blue) 3=STRONG (green) TITER: 1:2500
Click to enlarge

CPTC-GLO1-2 IHC NCI60

Immunohistochemistry of CPTC-GLO1-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
TITER: 1:2500

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GLO1-2. Titer: 1:2500
Click to enlarge

CPTC-GLO1-2 IHC Tissue

Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GLO1-2. Titer: 1:2500

Characterization SOP Files

IHC HPA

Download GLO-2 HPA Report.pdf (150.6 KB)

CPTC-GLO1-2 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Characterization SOP Files

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Antibody: CPTC-GLO1-3

Background

Catalog Number:
CPTC-GLO1-3
Target Antigen:
Lactoylglutathione lyase
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-GLO1-3 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-GLO1-3 Western Blot

Western Blot using CPTC-GLO1-3 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-GLO1-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-GLO1-3 Protein Array

Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

Click to enlarge image Immuno-histochemistry of CPTC-GLO1-3 for NCI60 Cell Line Array at titer 1:100 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green)
Click to enlarge

CPTC-GLO1-3 IHC NCI60

Immuno-histochemistry of CPTC-GLO1-3 for NCI60 Cell Line Array at titer 1:100
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for GLO1-3 Ab (615A28.1) using Biacore SPR method
Click to enlarge

CPTC-GLO1-3 SPR

Kinetic titration data for GLO1-3 Ab (615A28.1) using Biacore SPR method

Characterization SOP Files

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Antigen: Lactoylglutathione lyase

Background

NCI Identification Number:
10246
Antigen Name:
Lactoylglutathione lyase
CPTC Name:
CPTC-GLO1
Keywords:
GLO1; glyoxalase 1; Aldoketomutase; EC 4.4.1.5; GLOD1; GLY1; Methylglyoxalase; GLX1; ketone-aldehyde mutase; S-D-lactoyl glutathione methyl glyoxal lyase; OTTHUMP00000016339
Function:
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Chromosomal Localization:
6p21.3 p21.1
Expression System:
E. Coli
NCBI Accession Number:
NM_006708
Swiss Prot Accession Number:
Q04760
DNA Source:
HIP:HsCD00000982
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
25565
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQT
MLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFL
AYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDA
TQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKF
VKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Native Sequence:
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTML
RVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLA
YEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDA
TQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVK
FVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Calculated Isoelectric Point:
5.24
Molecular Weight:
20992
Last Updated:
04/12/2013

Links

Characterization Data

Gel

Click to enlarge image Kinetic titration data for GLO1-3 Ab (615A28.1) using Biacore SPR method
Click to enlarge

CPTC-GLO1 (Ag 10246) PAGE

SDS-PAGE of CPTC-GLO1 with molecular weight markers (in Lane 1)

SOPs

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