Catalog Number:
CPTC-GCLM-1
RRID:
AB_1553692
Target Antigen:
Glutamate-Cysteine Ligase Regulatory Subunit
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:20
Human assay: U-2 OS fixed with PFA, dilution: 1:20
Human assay: U-251 MG fixed with PFA, dilution: 1:20
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Western Blot Using CPTC-GCLM-1 as primary Ab against GCLM (Ag 10466)(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Catalog Number:
CPTC-GCLM-2
RRID:
AB_1553694
Target Antigen:
Glutamate-Cysteine Ligase Regulatory Subunit
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immunohistochemistry of CPTC-GCLM-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:100
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GCLM-2. Titer: 1:100
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GCLM-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot Using CPTC-GCLM-2 as primary Ab against GCLM (Ag 10466)(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Catalog Number:
CPTC-GCLM-3
RRID:
AB_2107843
Target Antigen:
Glutamate-Cysteine Ligase Regulatory Subunit
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-GCLM-3 for NCI60 Cell Line Array with titer of 1:5000
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GCLM-3. Titer: 1:5000
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GCLM-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Result: Positive
Western Blot Using CPTC-GCLM-3 as primary Ab against GCLM (Ag 10466)(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
NCI Identification Number:
10466
Antigen Name:
Glutamate-Cysteine Ligase Regulatory Subunit
CPTC Name:
CPTC-GCLM
Aliases:
GCLM; GLCLR; OTTHUMP00000012299
Function:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Chromosomal Localization:
1p22.1
Expression System:
E. Coli
Accession Number:
BC041809
UniProt Accession Number:
P48507
DNA Source:
HIP:HsCD00005436
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
39148
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCI
QKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFI
VESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYW
EELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMP
PDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWL
LRYSVIVKSRGIIKSKGYILQAKRRGS
Native Sequence:
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKT
LNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVES
NSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEEL
ENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDL
TAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRY
SVIVKSRGIIKSKGYILQAKRRGS
Calculated Isoelectric Point:
5.69
Molecular Weight:
30999
Last Updated:
09/01/2008
PAGE of GCLM (rAg 10466) with molecular weight standards in lane 1.
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