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Fascin

Antibody: CPTC-Fascin-1

Background

Catalog Number:
CPTC-Fascin-1
Target Antigen:
Fascin
Isotype:
IgG2a
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

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External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-Fascin-1 Western Blot

Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-Fascin-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antibody: CPTC-Fascin-2

Background

Catalog Number:
CPTC-Fascin-2
Target Antigen:
Fascin
Isotype:
IgG2a
Species:
Mouse
Last Updated:
03/07/2013
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-Fascin-2 Western Blot

Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-Fascin-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-Fascin-2 Protein Array

Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

CPTC-Fascin-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Array

IHC Tissue

CPTC-Fascin-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

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Antibody: CPTC-Fascin-3

Background

Catalog Number:
CPTC-Fascin-3
Target Antigen:
Fascin
Isotype:
IgG2b
Species:
Mouse
Last Updated:
08/28/2009
Antigen Recognition(s):
recombinant full-length

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-Fascin-3 Western Blot

Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-Fascin-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antigen: Fascin

Background

NCI Identification Number:
10411
Antigen Name:
Fascin
CPTC Name:
CPTC-Fascin
Keywords:
FSCN1; FAN1; FLJ38511; Fascin; HSN; SNL; p55
Function:
Chromosomal Localization:
7p22
Expression System:
E. Coli
NCBI Accession Number:
BC007948
Swiss Prot Accession Number:
Q16658
DNA Source:
HIP:HsCD00004052
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
68153
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPD
EAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHR
RYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAV
DRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKV
AFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQ
GMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNAS
CYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFR
GEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSG
DTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Native Sequence:
MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEA
GSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRR
YFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAV
DRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGK
VAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVST
RQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSK
NASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPI
IVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSA
VTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Calculated Isoelectric Point:
6.83
Molecular Weight:
54802
Last Updated:
04/09/2013

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-Fascin (rAg 10411) PAGE

PAGE of Fascin (rAg 10411) with molecular weight standards in lane 1.

SOPs

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