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Fatty acid-binding protein, epidermal

Antibody: CPTC-FABP5-1

Background

Catalog Number:
CPTC-FABP5-1
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-FABP5-1 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-FABP5-1 Western Blot

Western Blot using CPTC-FABP5-1 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP5-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

SPR

Download FABP5 pairing.pdf (793.1 KB)

Antibody Pairing Studies

This is a summary of antibody pairing studies by SPR.

Antibody: CPTC-FABP5-2

Background

Catalog Number:
CPTC-FABP5-2
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-FABP5-2 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-FABP5-2 Western Blot

Western Blot using CPTC-FABP5-2 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP5-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

SPR

Download FABP5 pairing.pdf (793.1 KB)

Antibody Pairing Studies

This summarizes antibody pairing studies by SPR.

Antibody: CPTC-FABP5-3

Background

Catalog Number:
CPTC-FABP5-3
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG2a
Species:
Mouse
Last Updated:
03/08/2012
Antigen Recognition(s):
recombinant full-length

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External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-FABP5-3 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-FABP5-3 Western Blot

Western Blot using CPTC-FABP5-3 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP5-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

SPR

Download FABP5 pairing.pdf (793.1 KB)

Antibody Pairing Studies

This summarizes antibody pairing studies by SPR.

Antigen: Fatty acid-binding protein, epidermal

Background

NCI Identification Number:
10280
Antigen Name:
Fatty acid-binding protein, epidermal
CPTC Name:
CPTC-FABP5
Keywords:
FABP5; E-FABP; EFABP; PA-FABP; PAFABP
Function:
This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
Chromosomal Localization:
8q21.13
Expression System:
E. Coli
NCBI Accession Number:
BC019385
Swiss Prot Accession Number:
Q01469
DNA Source:
HIP : HsCD00002133
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
14168
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMATVQQLEGRWRLVDSKGFDEYMKE
LGVGIALRKMGAMAKPDCIITCDGKNLT
IKTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTITR
KLKDGKLVVECVMNNVTCTRIYEKVE
Native Sequence:
MATVQQLEGRWRLVDSKGFDEYMKELG
VGIALRKMGAMAKPDCIITCDGKNLTI
KTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTIT
RKLKDGKLVVECVMNNVTCTRIYEKVE
Calculated Isoelectric Point:
6.57
Molecular Weight:
15437
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Ag 10280

PAGE of Ag 10280 with molecular weight standards in lane 1

SOPs

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