Fatty acid-binding protein, epidermal
Antibody: CPTC-FABP5-1
Background
Catalog Number:
CPTC-FABP5-1
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length
Purchase
- DSHB CPTC-FABP5-1
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-FABP5-1 Western Blot
Western Blot using CPTC-FABP5-1 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-FABP5-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
Download FABP5 pairing.pdf (793.1 KB)Antibody Pairing Studies
This is a summary of antibody pairing studies by SPR.
Characterization SOP Files
Antibody: CPTC-FABP5-2
Background
Catalog Number:
CPTC-FABP5-2
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length
Purchase
- DSHB CPTC-FABP5-2
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-FABP5-2 Western Blot
Western Blot using CPTC-FABP5-2 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-FABP5-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
Download FABP5 pairing.pdf (793.1 KB)Antibody Pairing Studies
This summarizes antibody pairing studies by SPR.
Characterization SOP Files
Antibody: CPTC-FABP5-3
Background
Catalog Number:
CPTC-FABP5-3
Target Antigen:
Fatty acid-binding protein, epidermal
Isotype:
IgG2a
Species:
Mouse
Last Updated:
03/08/2012
Antigen Recognition(s):
recombinant full-length
Purchase
- DSHB CPTC-FABP5-3
External Links
- Human Protein Atlas CPTC-FABP5-3
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-FABP5-3 Western Blot
Western Blot using CPTC-FABP5-3 as primary Ab against FABP5 (Ag 10280) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-FABP5-3 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
Download FABP5 pairing.pdf (793.1 KB)Antibody Pairing Studies
This summarizes antibody pairing studies by SPR.
Characterization SOP Files
Antigen: Fatty acid-binding protein, epidermal
Background
NCI Identification Number:
10280
Antigen Name:
Fatty acid-binding protein, epidermal
CPTC Name:
CPTC-FABP5
Keywords:
FABP5; E-FABP; EFABP; PA-FABP; PAFABP
Function:
This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
Chromosomal Localization:
8q21.13
Expression System:
E. Coli
NCBI Accession Number:
BC019385
Swiss Prot Accession Number:
Q01469
DNA Source:
HIP : HsCD00002133
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
14168
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMATVQQLEGRWRLVDSKGFDEYMKE
LGVGIALRKMGAMAKPDCIITCDGKNLT
IKTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTITR
KLKDGKLVVECVMNNVTCTRIYEKVE
LGVGIALRKMGAMAKPDCIITCDGKNLT
IKTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTITR
KLKDGKLVVECVMNNVTCTRIYEKVE
Native Sequence:
MATVQQLEGRWRLVDSKGFDEYMKELG
VGIALRKMGAMAKPDCIITCDGKNLTI
KTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTIT
RKLKDGKLVVECVMNNVTCTRIYEKVE
VGIALRKMGAMAKPDCIITCDGKNLTI
KTESTLKTTQFSCTLGEKFEETTADGR
KTQTVCNFTDGALVQHQEWDGKESTIT
RKLKDGKLVVECVMNNVTCTRIYEKVE
Calculated Isoelectric Point:
6.57
Molecular Weight:
15437
Last Updated:
11/14/2012
Links
- Uniprot - Uniprot FABP5
- EnsEMBL - EnsEMBL FABP5
- Entrez - Entrez FABP5
Characterization Data
SOPs
BL21 Competant Cells (25.5 KB)- High Throughput cloning MCSG7 (71.5 KB)
- High Throughput cloning MCSG19 (71.5 KB)
- Minimal Media Protein Growth (58.0 KB)
- Plasmid Purification (50.0 KB)
- PRK1037 Competent Cells (25.5 KB)
- Protein Purification using AKTA (76.5 KB)
- Cloning, Expression, Purification Flow Chart (37.5 KB)
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