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Fatty acid-binding protein, intestinal

Antibody: CPTC-FABP2-1

Background

Catalog Number:
CPTC-FABP2-1
Target Antigen:
Fatty acid-binding protein, intestinal
Isotype:
IgG1
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-FABP2-1 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-FABP2-1 Western blot

Western Blot using CPTC-FABP2-1 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP2-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-FABP2-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-FABP2-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-FABP2-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

IHC HPA

Download CPTC-FABP2-1.pdf (107.7 KB)

CPTC-FABP2-1

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antibody: CPTC-FABP2-2

Background

Catalog Number:
CPTC-FABP2-2
Target Antigen:
Fatty acid-binding protein, intestinal
Isotype:
IgG1
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-FABP2-2 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-FABP2-2 Western blot

Western Blot using CPTC-FABP2-2 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP2-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-FABP2-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-FABP2-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-FABP2-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

IHC HPA

Download CPTC-FABP2-2.pdf (95.3 KB)

CPTC-FABP2-2 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antigen: Fatty acid-binding protein, intestinal

Background

NCI Identification Number:
10977
Antigen Name:
Fatty acid-binding protein, intestinal
CPTC Name:
CPTC-FABP2
Keywords:
FABPI; I-FABP; MGC133132
Function:
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids.

They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis.

Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Chromosomal Localization:
4q28 - q31
Expression System:
E. Coli
NCBI Accession Number:
BC069625.1
Swiss Prot Accession Number:
P12104
DNA Source:
OB : MHS1010-98684774
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
16960
Buffers:
PBS
Expressed Sequence:
SNAMAFDSTWKVDRSENYDKFMEKMGV
NIVKRKLAAHDNLKLTITQEGNKFTVK
ESSAFRNIEVVFELGVTFNYNLADGTE
LRGTWSLEGNKLIGKFKRTDNGNELNT
VREIIGDELVQTYVYEGVEAKRIFKKD
Native Sequence:
MAFDSTWKVDRSENYDKFMEKMGVNIV
KRKLAAHDNLKLTITQEGNKFTVKESS
AFRNIEVVFELGVTFNYNLADGTELRG
TWSLEGNKLIGKFKRTDNGNELNTVRE
IIGDELVQTYVYEGVEAKRIFKKD
Calculated Isoelectric Point:
6.62
Molecular Weight:
15.21
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-FABP2 PAGE

PAGE of Ag 10977 (with molecular weight standards in lane 1)

SOPs

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