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Cystatin B

Antibody: CPTC-CSTB-1

Background

Catalog Number:
CPTC-CSTB-1
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/24/2013
Antigen Recognition(s):

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTB-1 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTB-1 Western blot

Western Blot using CPTC-CSTB-1 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTB-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC HPA

Download CPTC-CSTB-1.pdf (88.7 KB)

CPTC-CSTB-1 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antibody: CPTC-CSTB-2

Background

Catalog Number:
CPTC-CSTB-2
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/24/2013
Antigen Recognition(s):

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTB-2 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTB-2 Western blot

Western Blot using CPTC-CSTB-2 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTB-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC HPA

Download CPTC-CSTB-2.pdf (86.7 KB)

CPTC-CSTB-2 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antibody: CPTC-CSTB-3

Background

Catalog Number:
CPTC-CSTB-3
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/24/2013
Antigen Recognition(s):

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTB-3 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTB-3 Western blot

Western Blot using CPTC-CSTB-3 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTB-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC HPA

Download CPTC-CSTB-3.pdf (99.5 KB)

CPTC-CSTB-3 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antigen: Cystatin B

Background

NCI Identification Number:
10098
Antigen Name:
Cystatin B
CPTC Name:
CPTC-CSTB
Keywords:
CPI-B; CST6; EPM1; PME; STFB; Stefin-B
Function:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins L, H and B. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B
Chromosomal Localization:
21q22.3
Expression System:
E. Coli
NCBI Accession Number:
BC010532
Swiss Prot Accession Number:
P04080
DNA Source:
HIP : HsCD00002240
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
4470
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMMCGAPSATQPATAETQH
IADQVRSQLEEKENKKFPVFK
AVSFKSQVVAGTNYFIKVHVG
DEDFVHLRVFQSLPHENKPLT
LSNYQTNKAKHDELTYF
Native Sequence:
MMCGAPSATQPATAETQHIA
DQVRSQLEEKENKKFPVFKA
VSFKSQVVAGTNYFIKVHVG
DEDFVHLRVFQSLPHENKPL
TLSNYQTNKAKHDELTYF
Calculated Isoelectric Point:
6.94
Molecular Weight:
11412
Last Updated:
02/26/2013

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTB SDS-PAGE

PAGE of CSTB (Ag 10098) with molecular weight standards in lane 1

SOPs

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