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Cystatin A

Antibody: CPTC-CSTA-1

Background

Catalog Number:
CPTC-CSTA-1
Target Antigen:
Cystatin A
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTA-1 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTA-1 Western blot

Western Blot using CPTC-CSTA-1 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTA-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-CSTA-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-CSTA-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-CSTA-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

IHC HPA

Download CPTC-CSTA-1.pdf (105.7 KB)

CPTC-CSTA-1 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antibody: CPTC-CSTA-2

Background

Catalog Number:
CPTC-CSTA-2
Target Antigen:
Cystatin A
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/26/2013
Antigen Recognition(s):

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTA-2 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTA-2 Western blot

Western Blot using CPTC-CSTA-2 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTA-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-CSTA-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CSTA-CSTA-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CSTA-CSTA-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

IHC HPA

Download CPTC-CSTA-2.pdf (99.5 KB)

CSTA-CSTA-2 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antibody: CPTC-CSTA-3

Background

Catalog Number:
CPTC-CSTA-3
Target Antigen:
Cystatin A
Isotype:
IgG1
Species:
Mouse
Last Updated:
03/07/2013
Antigen Recognition(s):

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CSTA-3 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Click to enlarge

CPTC-CSTA-3 Western blot

Western Blot using CPTC-CSTA-3 as primary Ab against rAg 10991 (CSTA) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-CSTA-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-CSTA-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-CSTA-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Array

IHC Tissue

CPTC-CSTA-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

IHC HPA

Download CPTC-CSTA-3.pdf (96.0 KB)

CPTC-CSTA-3 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Characterization SOP Files

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Antigen: Cystatin A

Background

NCI Identification Number:
10991
Antigen Name:
Cystatin A
CPTC Name:
CPTC-CSTA
Keywords:
Cystatin-AS; STF1; STFA; Stefin-A
Function:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. This is an intracellular thiol proteinase inhibitor.
Chromosomal Localization:
3q21
Expression System:
E. Coli
NCBI Accession Number:
BC010379.1
Swiss Prot Accession Number:
P01040
DNA Source:
OB : MHS1011-74586
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
8940
Buffers:
PBS
Expressed Sequence:
SNAMIPGGLSEAKPATPEIQE
IVDKVKPQLEEKTNETYGKLE
AVQYKTQVVAGTNYYIKVRAG
DNKYMHLKVFKSLPGQNEDLV
LTGYQVDKNKDDELTGF
Native Sequence:
MIPGGLSEAKPATPEIQEIV
DKVKPQLEEKTNETYGKLEA
VQYKTQVVAGTNYYIKVRAG
DNKYMHLKVFKSLPGQNEDL
VLTGYQVDKNKDDELTGF
Calculated Isoelectric Point:
5.38
Molecular Weight:
11.01
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-Cystatin A PAGE

PAGE of Ag 10991 (with molecular weight standards in lane 1)

SOPs

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