Crystallin Alpha B
Background
Purchase
- DSHB CPTC-CRYAB-1
External Links
- Human Protein Atlas CPTC-CRYAB-1
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-CRYAB-1 Western Blot
Western Blot using CPTC-CRYAB-1 as primary Ab against Ag 10408 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-CRYAB-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-CRYAB-1 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-CRYAB-1 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-CRYAB-1 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NAPPA
Download CRYAB-1 pos.pdf (1.1 MB)Evaluation of antibody by NAPPA
This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.
Characterization SOP Files
Background
Purchase
- DSHB CPTC-CRYAB-2
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-CRYAB-2 Western Blot
Western Blot using CPTC-CRYAB-2 as primary Ab against Ag 10408 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-CRYAB-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-CRYAB-2 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-CRYAB-2 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-CRYAB-2 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NAPPA
Download CRYAB-2 pos.pdf (855.8 KB)Evaluation of antibody by NAPPA
This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.
Characterization SOP Files
Background
Purchase
- DSHB CPTC-CRYAB-3
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-CRYAB-3 Western Blot
Western Blot using CPTC-CRYAB-3 as primary Ab against Ag 10408 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-CRYAB-3 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-CRYAB-3 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-CRYAB-3 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-CRYAB-3 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Background
class constitutes the major proteins of vertebrate eye lens and maintains the transparency and
refractive index of the lens. Since lens central fiber cells lose their nuclei during development,
these crystallins are made and then retained throughout life, making them extremely stable
proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and
gamma crystallins are also considered as a superfamily. Alpha and beta families are further
divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.
Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two
additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed;
alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues
and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a
missense mutation cosegregated in a family with a desmin-related myopathy.
ESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMR
LEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHE
ERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDG
VLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK
DLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRL
EKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHE
ERQDEHGFISREFHRKYRIPADVDPLTITSSLSSD
GVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK
Links
- Uniprot - Uniprot CRYAB
- EnsEMBL - EnsEMBL CRYAB
- Entrez - Entrez CRYAB
Characterization Data
SOPs
Adobe Reader™ required.
Get Adobe Reader for free at Adobe.com