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Clusterin Peptide 1

Antibody: CPTC-CLU-1

Background

Catalog Number:
CPTC-CLU-1
Target Antigen:
Clusterin Peptide 1
Isotype:
IgG
Species:
Rabbit
Last Updated:
04/02/2015
Antigen Recognition(s):
peptide, recombinant full-length, native protein

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-CLU-1 as primary Ab against HEK293T cell lysate containing CLU (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CLU-1 Ab as the IgG control (lane 3).
Click to enlarge

CPTC-CLU-1 Western blot (cell lysate)

Western Blot using CPTC-CLU-1 as primary Ab against HEK293T cell lysate containing CLU (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CLU-1 Ab as the IgG control (lane 3).

Cell Lysate Western Blot

Click to enlarge image Western Blot using CPTC-CLU-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).
Click to enlarge

CPTC-CLU-1 NCI60 Western blot

Western Blot using CPTC-CLU-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-CLU-1 Indirect ELISA (peptide)

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-CLU-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Antigen: Clusterin Peptide 1

Background

NCI Identification Number:
00053
Antigen Name:
Clusterin Peptide 1
CPTC Name:
CPTC-CLU Peptide 1
Keywords:
CLI; APOJ1; KUB1; TRPM-2; CLU1; CLU2; SGP-2; SP-40; Apolipoprotein J; Complement Cytolysis Inhibitor; Complement-Associated Protein SP-40,40; Ku70-Binding Protein 1; Testosterone-Repressed Prostate Message 2; NA1/NA2; SGP2; TRPM2; Clusterin (Complement Lysis Inhibitor, SP-40,40; Clusterin (Complement Lysis Inhibitor, SP-40,40, Sulfated Glycoprotein 2,Testosterone-Repressed Prostate Message 2, Apolipoprotein J); APO-J; Aging-Associated Protein 4; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Aging-Associated Gene 4 Protein
Function:
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the
cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor
progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variant.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA,
CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by
complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein
ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote
apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Chromosomal Localization:
8p21-p12
Expression System:
NCBI Accession Number:
NP_001822.3
Swiss Prot Accession Number:
P10909
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
ASSIIDELFQDR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1320
Last Updated:
06/21/2013

Links

Characterization Data

SOPs

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