Cell division cycle 34 homolog (S. Cerevisiae)
Antibody: CPTC-CDC34-1
Background
Catalog Number:
CPTC-CDC34-1
Target Antigen:
Cell division cycle 34 homolog (S. Cerevisiae)
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/24/2013
Antigen Recognition(s):
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-CDC34-1 Western blot
Western Blot using CPTC-CDC34-1 as primary Ab against Ag 10253 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-CDC34-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
IHC HPA
Download CPTC-CDC34-1.pdf (103.7 KB)CPTC-CDC34-1 Evaluation by the Human Protein Atlas
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Antibody: CPTC-CDC34-2
Background
Catalog Number:
CPTC-CDC34-2
Target Antigen:
Cell division cycle 34 homolog (S. Cerevisiae)
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/24/2013
Antigen Recognition(s):
External Links
- Human Protein Atlas CPTC-CDC34-2
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
CPTC-CDC34-2 Western blot
Western Blot using CPTC-CDC34-2 as primary Ab against Ag 10253 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-CDC34-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
IHC HPA
Download CPTC-CDC34-2.pdf (114.9 KB)CPTC-CDC34-2 Evaluation by the Human Protein Atlas
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Antigen: Cell division cycle 34 homolog (S. Cerevisiae)
Background
NCI Identification Number:
10253
Antigen Name:
Cell division cycle 34 homolog (S. Cerevisiae)
CPTC Name:
CPTC-CDC34
Keywords:
CDC34; UBC3; UBE2R1; E2-CDC34; EC 6.3.2.19
Function:
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins.
This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.
This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.
Chromosomal Localization:
19p13.3
Expression System:
E. Coli
NCBI Accession Number:
BC018143
Swiss Prot Accession Number:
P49427
DNA Source:
HIP : HsCD00002069
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG19
Extinction Coefficient:
41433
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAI
FGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVC
ISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPAN
VDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAE
YCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
FGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVC
ISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPAN
VDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAE
YCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
Native Sequence:
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGP
PNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISI
LHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDA
SVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCV
KTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
PNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISI
LHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDA
SVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCV
KTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
Calculated Isoelectric Point:
4.41
Molecular Weight:
27009
Last Updated:
11/14/2012
Links
- Uniprot - Uniprot CDC34
- EnsEMBL - EnsEMBL CDC34
- Entrez - Entrez CDC34
Characterization Data
SOPs
BL21 Competant Cells (25.5 KB)- High Throughput cloning MCSG7 (71.5 KB)
- High Throughput cloning MCSG19 (71.5 KB)
- Minimal Media Protein Growth (58.0 KB)
- Plasmid Purification (50.0 KB)
- PRK1037 Competent Cells (25.5 KB)
- Protein Purification using AKTA (76.5 KB)
- Cloning, Expression, Purification Flow Chart (37.5 KB)
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