BCL2 like 1
Background
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- DSHB CPTC-BCL2L1-1
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-BCL2L1-1 Western blot
Western Blot using CPTC-BCL2L1-1 as primary Ab against BCL2L1 (rAg 10650) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-BCL2L1-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-BCL2L1-1 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-BCL2L1-1 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
IHC Tissue
CPTC-BCL2L1-1 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
SPR
Download BCL2L1 pairing.pdf (791.8 KB)SPR pairing results
These are the results of SPR pairing experiments with BCL2L1 antibodies.
Background
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- DSHB CPTC-BCL2L1-2
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-BCL2L1-2 Western blot
Western Blot using CPTC-BCL2L1-2 as primary Ab against BCL2L1 (rAg 10650) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-BCL2L1-2 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-BCL2L1-2 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-BCL2L1-2 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
IHC Tissue
CPTC-BCL2L1-2 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
SPR
Download BCL2L1 pairing.pdf (791.8 KB)SPR pairing results
These are the results of SPR pairing experiments with BCL2L1 antibodies.
Background
Purchase
- DSHB CPTC-BCL2L1-3
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-BCL2L1-3 Western blot
Western Blot using CPTC-BCL2L1-3 as primary Ab against BCL2L1 (rAg 10650) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
CPTC-BCL2L1-3 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-BCL2L1-3 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-BCL2L1-3 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
IHC Tissue
CPTC-BCL2L1-3 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
SPR
Download BCL2L1 pairing.pdf (791.8 KB)SPR pairing results
These are the results of SPR pairing experiments with BCL2L1 antibodies.
Background
death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the
caspase activator, CYC1, from the mitochondrial membrane
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the
production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.
Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. The pro-apoptotic group of Bcl-2 proteins can be further sub-divided into the structurally diverse 'BH3' only proteins (e.g. Bid, Noxa, Puma and Bad) and the multidomain proteins that share BH1 to 3 (e.g. Bax and Bak). Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes.
TESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPM
AAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQV
VNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAW
MATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERF
PSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAG
DEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIV
AFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGW
DTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
Links
Characterization Data
Gel
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CPTC-BCL2L1 PAGE
PAGE of BCL2L2 (rAg 10650) with molecular weight standards in lane 1
SOPs
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