Background
Catalog Number:
CPTC-AKR1C1-1
RRID:
AB_2617218
Target Antigen:
Aldo-keto Reductase Family 1 Member C1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/24/2019
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-AKR1C1-1
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
Cross Reactivity Data
Result: Positive
This table shows cross reactivity within the AKR1 family of antigens.
Characterization SOP Files
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (87.5 KB)
CPTC-AKR1C1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-AKR1C1-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-AKR1C1-1 Western blot
Result: Positive
Western Blot using CPTC-AKR1C1-1 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-AKR1C1-2
Target Antigen:
Aldo-keto Reductase Family 1 Member C1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-AKR1C1-2
External Links
- Human Protein Atlas Human Protein Atlas CPTC-AKR1C1-2
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
Cross Reactivity Data
Result: Positive
This table shows cross reactivity within the AKR1 family of antigens.
Characterization SOP Files
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (118.2 KB)
CPTC-AKR1C1-2 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
CPTC-AKR1C1-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & endoplasmic reticulum. Human assay: Hep G2 fixed with PFA, dilution: 1:175.
Characterization SOP Files
Indirect ELISA
CPTC-AKR1C1-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-AKR1C1-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-AKR1C1-2 Western blot
Result: Positive
Western Blot using CPTC-AKR1C1-2 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10013
Antigen Name:
Aldo-keto Reductase Family 1 Member C1
CPTC Name:
CPTC-AKR1C1
Aliases:
AKR1C1; 2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DD1/DD2; DDH; DDH1; H-37; EC 1.1.1.-,EC 1.1.1.149,EC 1.3.1.20; HAKRC; HBAB; MBAB; MGC8954; OTTHUMP00000018992
Function:
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone(20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May
play a role in myelin formation.
Chromosomal Localization:
10p15-p14
Expression System:
E. Coli
Accession Number:
NM_001353
UniProt Accession Number:
Q04828
DNA Source:
INV : 4662654
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
37610
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEAGFR
HIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRP
ALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCAT
WEAVEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYF
NQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKK
HKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAID
GLNRNVRYLTLDIFAGPPNYPFSDEY
Native Sequence:
MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEAGFRHID
SAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRPALE
RSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEA
VEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNQR
KLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKR
TPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLN
RNVRYLTLDIFAGPPNYPFSDEY
Calculated Isoelectric Point:
8.01
Molecular Weight:
37061
Last Updated:
09/01/2008
Links
- EnsEMBL - EnsEMBL AKR1C1
- Entrez - Entrez AKR1C1
- Uniprot - Uniprot Q04828
Characterization Data
Gel
Click image to enlarge
Ag 10013
PAGE of Ag 10013 (with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
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