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Aldo-keto Reductase Family 1 Member B1

Antibody: CPTC-AKR1B1-1

Background

Catalog Number:
CPTC-AKR1B1-1
Target Antigen:
Aldo-keto Reductase Family 1 Member B1
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-1 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-1 Western Blot

Western Blot using CPTC-AKR1B1-1 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Cell Lysate Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-1 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-1 Cell Lysate blot

Western Blot using CPTC-AKR1B1-1 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-AKR1B1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens.
Click to enlarge

Cross Reactivity Data

This table shows cross reactivity within the AKR1 family of antigens.

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression (red).
Click to enlarge

CPTC-AKR1B1-1 NCI 60 Protein Array

Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression (red).

Characterization SOP Files

IHC NCI60

CPTC-AKR1B1-1 IHC NCI60

Immuno-histochemistry of CPTC-AKR1B1-1 for NCI60 Cell Line Array at titer 1:500
0=NEGATIVE
1=WEAK(RED)
2=MODERATE(BLUE)
3=STRONG(GREEN)

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-AKR1B1-1. Titer: 1:500
Click to enlarge

CPTC-AKR1B1-1 IHC Tissue

Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-AKR1B1-1. Titer: 1:500

Characterization SOP Files

IHC HPA

Download AKR1B1-1.pdf (464.3 KB)

CPTC-AKR1B1-1 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Characterization SOP Files

Immuno-MS

Download AKR1B1-1.pdf (604.7 KB)

CPTC-AKR1B1-1 Immuno MS

This PDF gives the results of Immuno MS with CPTC-AKR1B1-1 and the AKR1B1 antigen.

Characterization SOP Files

NAPPA

Download AKR1B1-1.pdf (163.0 KB)

Evaluation of antibody by NAPPA

This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.

Characterization SOP Files

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Antibody: CPTC-AKR1B1-2

Background

Catalog Number:
CPTC-AKR1B1-2
Target Antigen:
Aldo-keto Reductase Family 1 Member B1
Isotype:
IgG1
Species:
Mouse
Last Updated:
03/07/2013
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-2 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-2 Western Blot

Western Blot using CPTC-AKR1B1-2 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Cell Lysate Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-2 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-2 Cell Lysate Western Blot

Western Blot using CPTC-AKR1B1-2 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-AKR1B1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens.
Click to enlarge

Cross Reactivity Data

This table shows cross reactivity within the AKR1 family of antigens.

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-AKR1B1-2 NCI 60 Protein Array

Protein Array in which CPTC-AKR1B1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

CPTC-AKR1B1-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Array

IHC Tissue

CPTC-AKR1B1-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

Immuno-MS

Download AKR1B1-2.pdf (593.8 KB)

CPTC-AKR1B1-2 Immuno MS

This PDF provides the results of Immuno MS with CPTC-AKR1B1-2 antibody and AKR1B1 antigen.

Characterization SOP Files

NAPPA

Download AKR1B1-2.pdf (1.2 MB)

Evaluation of antibody by NAPPA

This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.

Characterization SOP Files

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Antibody: CPTC-AKR1B1-3

Background

Catalog Number:
CPTC-AKR1B1-3
Target Antigen:
Aldo-keto Reductase Family 1 Member B1
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/15/2011
Antigen Recognition(s):
recombinant full-length

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-3 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-3 Western Blot

Western Blot using CPTC-AKR1B1-3 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Cell Lysate Western Blot

Click to enlarge image Western Blot using CPTC-AKR1B1-3 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-AKR1B1-3 Cell Lysate Western Blot

Western Blot using CPTC-AKR1B1-3 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-AKR1B1-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

Cross Reactivity Data

Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens.
Click to enlarge

Cross Reactivity Data

This table shows cross reactivity within the AKR1 family of antigens.

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-AKR1B1-3 Protein Array

Protein Array in which CPTC-AKR1B1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC NCI60

CPTC-AKR1B1-3 IHC NCI60

Immuno-histochemistry of CPTC-AKR1B1-3 for NCI60 Cell Line Array at titer 1:250
0=NEGATIVE
1=WEAK
2=MODERATE
3=STRONG

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-AKR1B1-3. Titer: 1:250
Click to enlarge

CPTC-AKR1B1-3 IHC Tissue

Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-AKR1B1-3. Titer: 1:250

Characterization SOP Files

IHC HPA

Download AKR1B1-3 HPA Report.pdf (139.7 KB)

CPTC-AKR1B1-3 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Characterization SOP Files

Immuno-MS

Download AKR1B1-3.pdf (593.9 KB)

CPTC-AKR1B1-3 Immuno MS

This PDF provides the results of Immuno MS with CPTC-AKR1B1-3 antibody and AKR1B1 antigen.

Characterization SOP Files

NAPPA

Download AKR1B1-3.pdf (1.2 MB)

Evaluation of antibody by NAPPA

This antibody was found to be unsuitable for evaluation by NAPPA because of low reactivity above background.

Characterization SOP Files

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Antigen: Aldo-keto Reductase Family 1 Member B1

Background

NCI Identification Number:
10316
Antigen Name:
Aldo-keto Reductase Family 1 Member B1
CPTC Name:
CPTC-AKR1B1
Keywords:
AKR1B1; ADR; ALDR1; ALR2; AR; EC 1.1.1.21; MGC1804
Function:
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic
complications by catalyzing the reduction of glucose to sorbitol. There are a few putative
pseudogenes for this gene, and one of them has been confirmed and mapped to chromosome 3.

It functions to catalyze the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Chromosomal Localization:
7q35
Expression System:
E. Coli
NCBI Accession Number:
BC000260
Swiss Prot Accession Number:
P15121
DNA Source:
HIP:HsCD00003028
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
49578
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQE
KLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPL
DESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHP
YLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFP
MQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF
Native Sequence:
MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLR
EQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDES
GNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLT
QEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQR
NLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF
Calculated Isoelectric Point:
6.51
Molecular Weight:
36126
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-AKR1B1-3. Titer: 1:250
Click to enlarge

Ag 10316

PAGE of Ag 10316 with molecular weight standards

SOPs

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