U.S. National Institutes of Health | www.cancer.gov

CPTC Reagents Data Portal

14-3-3 sigma

Go | Expand All - Collapse All
Antibodies
Antigen
Antibody: CPTC-SFN-1
View Antibody Record

Background

Catalog Number:
CPTC-SFN-1
Target Antigen:
14-3-3 sigma
Isotype:
IgG1
Species:
Mouse
Last Updated:
08/28/2009

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-SFN-1 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-SFN-1 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

[top]
Antibody: CPTC-SFN-2
View Antibody Record

Background

Catalog Number:
CPTC-SFN-2
Target Antigen:
14-3-3 sigma
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/19/2010

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-SFN-2 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-SFN-2 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC NCI60

Download 636A16.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-SFN-2 for NCI60 Cell Line Array at titer 1:250 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-SFN-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-SFN-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

[top]
Antibody: CPTC-SFN-3
View Antibody Record

Background

Catalog Number:
CPTC-SFN-3
Target Antigen:
14-3-3 sigma
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/14/2010

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-SFN-3 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-SFN-3 as primary Ab against Ag 10467 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC NCI60

Download 636A5.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-SFN-3 for NCI60 Cell Line Array at titer 1:500 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-SFN-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-SFN-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

[top]
Antigen: 14-3-3 sigma
View Anitgen Record

Background

NCI Identification Number:
10467
Antigen Name:
14-3-3 sigma
CPTC Name:
CPTC-SFN
Keywords:
SFN; HME1; Stratifin; OTTHUMP00000004242; YWHAS;
Function:
p53-regulated inhibitor of G2/M progression
Chromosomal Localization:
1p36.11
Expression System:
E. Coli
NCBI Accession Number:
BC000995
Swiss Prot Accession Number:
P31947
DNA Source:
HIP:HsCD00005964
Vector Name:
pMCSG7
Extinction Coefficient:
25963
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAY
KNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTV
LGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSA
YQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEA
MADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS
Native Sequence:
MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKN
VVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVL
GLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSA
YQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDE
AMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS
Calculated Isoelectric Point:
4.68
Molecular Weight:
28046
Last Updated:
04/06/2009

Links

Characterization Data

Gel

Click to enlarge image Protein Array in which CPTC-SFN-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-SFN PAGE

PAGE of Ag 10467 (CPTC-SFN) (with molecular weight standards in lane 1)

Characterization SOP Files

SOPs

[top]

Adobe Reader™ required.

Get Adobe Reader for free at Adobe.com

A Service of the National Cancer Institute
Site by The Advanced Biomedical Computing Center (ABCC)