U.S. National Institutes of Health | www.cancer.gov

CPTC Reagents Data Portal

Metastasin 100 calcium-binding protein A4 (Calvasculin)

Go | Expand All - Collapse All
Antibodies
Antigen
Antibody: CPTC-S100A4-1
View Antibody Record

Background

Catalog Number:
CPTC-S100A4-1
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/25/2010

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-1 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-S100A4-1 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-1
Click to enlarge

Indirect ELISA of CPTC-S100A4-1

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for S100A4-1 Ab (635A8.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for S100A4-1 Ab (635A8.1) using Biacore SPR method

Characterization SOP Files

Immuno-MS

Download S100A4-1.pdf (200.3 KB)

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which S100A4-1 is screen agains the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Click to enlarge

Protein Array in which S100A4-1 is screen agains the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.

Characterization SOP Files

[top]
Antibody: CPTC-S100A4-2
View Antibody Record

Background

Catalog Number:
CPTC-S100A4-2
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/25/2010

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-2 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-S100A4-2 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-2
Click to enlarge

Indirect ELISA of CPTC-S100A4-2

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for S100A4-2 Ab (635A11.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for S100A4-2 Ab (635A11.1) using Biacore SPR method

Characterization SOP Files

IHC NCI60

Download 635A11.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-S100A4-2 for NCI60 Cell Line Array at titer 1:8000 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

Immuno-MS

Download S100A4-2.pdf (199.1 KB)

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC HPA

Download S100A4-2 HPA Report.pdf (558.4 KB)

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Characterization SOP Files

[top]
Antibody: CPTC-S100A4-3
View Antibody Record

Background

Catalog Number:
CPTC-S100A4-3
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/25/2010

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-3 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-S100A4-3 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-3
Click to enlarge

Indirect ELISA of CPTC-S100A4-3

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for S100A4-3 Ab (635A23.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for S100A4-3 Ab (635A23.1) using Biacore SPR method

Characterization SOP Files

IHC NCI60

Download Metastasin 100 635A23.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-S100A4-3 for NCI60 Cell Line Array 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

Immuno-MS

Download S100A4-3.pdf (202.1 KB)

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-S100A4-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-S100A4-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

IHC HPA

Download S100A4-3 HPA Report.pdf (161.4 KB)

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Characterization SOP Files

[top]
Antigen: Metastasin 100 calcium-binding protein A4 (Calvasculin)
View Anitgen Record

Background

NCI Identification Number:
10337
Antigen Name:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
CPTC Name:
CPTC-S100A4
Keywords:
S100A4; 18A2; 42A; CAPL; Calvasculin; FSP1; MTS1; Metastasin; OTTHUMP00000015467; OTTHUMP00000015468; OTTHUMP00000015469; OTTHUMP00000032895; P9KA; PEL98
Function:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Chromosomal Localization:
1q21
Expression System:
E. Coli
NCBI Accession Number:
M80563
Swiss Prot Accession Number:
P26447
DNA Source:
HIP:HsCD00004965
Vector Name:
pMCSG7
Extinction Coefficient:
3105
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMACPLEKALDVMVSTFHK
YSGKEGDKFKLNKSELKELLT
RELPSFLGKRTDEAAFQKLMS
NLDSNRDNEVDFQEYCVFLSC
IAMMCNEFFEGFPDKQPRKK
Native Sequence:
MACPLEKALDVMVSTFHKYSG
KEGDKFKLNKSELKELLTREL
PSFLGKRTDEAAFQKLMSNLD
SNRDNEVDFQEYCVFLSCIAM
MCNEFFEGFPDKQPRKK
Calculated Isoelectric Point:
5.84
Molecular Weight:
12001
Last Updated:
01/28/2009

Links

Characterization Data

Gel

Click to enlarge image Protein Array in which CPTC-S100A4-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Ag 10337

PAGE of Ag 10337 with molecular weight standards

Characterization SOP Files

SOPs

[top]

Adobe Reader™ required.

Get Adobe Reader for free at Adobe.com

A Service of the National Cancer Institute
Site by The Advanced Biomedical Computing Center (ABCC)