Protein Phosphatase 2A
Background
Purchase
- Purchase CPTC-PP2A-1 (Protein Phosphatase 2A-1) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
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Western Blot using CPTC-PP2A-1 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
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Kinetic titration data for PP2A-1 Ab (622A1.1) using Biacore SPR method
Characterization SOP Files
Background
Purchase
- Purchase CPTC-PP2A-2 (Protein Phosphatase 2A-2) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
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Western Blot using CPTC-PP2A-2 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
SPR
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Kinetic titration data for PP2A-2 Ab (622A3.1) using Biacore SPR method
Characterization SOP Files
Background
Purchase
- Purchase CPTC-PP2A-3 (Protein Phosphatase 2A-3) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
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Western Blot using CPTC-PP2A-3 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protien Array
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Protein Array in which CPTC-PP2A-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
Purchase
- Purchase CPTC-PP2A-4 (Protein Phosphatase 2A-4) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
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Western Blot using CPTC-PP2A-4 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Background
Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than
the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen
LTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRT
WYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAF
LCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQF
LPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHS
NQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG
LNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTW
YAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAF
LCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQ
FLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAE
HSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG
Links
- Uniprot - Uniprot PP2A
- EnsEMBL - EnsEMBL PP2A
- Entrez - Entrez PP2A
Characterization Data
Gel
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CPTC-PP2A (Ag 10501) PAGE
PAGE of Ag 10501 with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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