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CPTC Reagents Data Portal

Nucleoside Diphosphate Kinase B

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Antibodies
Antigen
Antibody: CPTC-NME2-1
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Background

Catalog Number:
CPTC-NME2-1
Target Antigen:
Nucleoside Diphosphate Kinase B
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/25/2010

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-NME2-1
Click to enlarge

Indirect ELISA of CPTC-NME2-1

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method

Characterization SOP Files

Immuno-MS

Download NME2-1.pdf (123.0 KB)

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

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Antibody: CPTC-NME2-2
View Antibody Record

Background

Catalog Number:
CPTC-NME2-2
Target Antigen:
Nucleoside Diphosphate Kinase B
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/25/2010

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-NME2-2
Click to enlarge

Indirect ELISA of CPTC-NME2-2

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method

Characterization SOP Files

Immuno-MS

Download NME2-2.pdf (118.8 KB)

This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-NME2-3
View Antibody Record

Background

Catalog Number:
CPTC-NME2-3
Target Antigen:
Nucleoside Diphosphate Kinase B
Isotype:
IgG2b
Species:
Mouse
Last Updated:
01/25/2010

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-NME2-3
Click to enlarge

Indirect ELISA of CPTC-NME2-3

Characterization SOP Files

SPR

Click to enlarge image Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method
Click to enlarge

Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method

Characterization SOP Files

Immuno-MS

Download NME2-3.pdf (125.8 KB)

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Characterization SOP Files

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Antigen: Nucleoside Diphosphate Kinase B
View Anitgen Record

Background

NCI Identification Number:
10385
Antigen Name:
Nucleoside Diphosphate Kinase B
CPTC Name:
CPTC-NME2
Keywords:
Nucleoside Diphosphate Kinase B; NME2; NM23B; EC2.7.4.6; MGC111212; NDPKB; NM23-H2;
Function:
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC.

Acts as a transcriptional activator of the c-Myc gene; binds DNA nonspecifically
Chromosomal Localization:
17q21 - q22
Expression System:
E. Coli
NCBI Accession Number:
BC002476
Swiss Prot Accession Number:
P22392
DNA Source:
HIP:HsCD00002962
Vector Name:
pMCSG7
Extinction Coefficient:
22523
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMANLERTFIAIKPDGVQRGLVGEIIKRF
EQKGFRLVAMKFLRASEEHLKQHYIDLKDRP
FFPGLVKYMNSGPVVAMVWEGLNVVKTGRVM
LGETNPADSKPGTIRGDFCIQVGRNIIHGSD
SVKSAEKEISLWFKPEELVDYKSCAHDWVYE
Native Sequence:
MANLERTFIAIKPDGVQRGLVGEIIKRFEQK
GFRLVAMKFLRASEEHLKQHYIDLKDRPFFP
GLVKYMNSGPVVAMVWEGLNVVKTGRVMLGE
TNPADSKPGTIRGDFCIQVGRNIIHGSDSVK
SAEKEISLWFKPEELVDYKSCAHDWVYE
Calculated Isoelectric Point:
8.52
Molecular Weight:
17570
Last Updated:
03/03/2009

Links

Characterization Data

Gel

Click to enlarge image Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method
Click to enlarge

PAGE of ANL antigen 10385

Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.

Characterization SOP Files

SOPs

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