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CPTC Reagents Data Portal

Melanoma Antigen Family A, 4

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Antibodies
Antigen
Antibody: CPTC-MAGEA4-1
View Antibody Record

Background

Catalog Number:
CPTC-MAGEA4-1
Target Antigen:
Melanoma Antigen Family A, 4
Isotype:
IgG2a
Species:
Mouse
Last Updated:
09/23/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-MAGEA4-1 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-MAGEA4-1 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC NCI60

Download 614A3.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-MAGEA4-1 for NCI60 Cell Line Array at titer 1:100 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-MAGEA4-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Click to enlarge

Protein Array in which CPTC-MAGEA4-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.

Characterization SOP Files

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Antibody: CPTC-MAGEA4-2
View Antibody Record

Background

Catalog Number:
CPTC-MAGEA4-2
Target Antigen:
Melanoma Antigen Family A, 4
Isotype:
IgG2b
Species:
Mouse
Last Updated:
12/23/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-MAGEA4-2 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-MAGEA4-2 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC NCI60

Download 614A32.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-MAGEA4-2 for NCI60 Cell Line Array at titer 1:1250 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-MAGEA4-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-MAGEA4-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-MAGEA4-3
View Antibody Record

Background

Catalog Number:
CPTC-MAGEA4-3
Target Antigen:
Melanoma Antigen Family A, 4
Isotype:
IgG1
Species:
Mouse
Last Updated:
06/17/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-MAGEA4-3 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-MAGEA4-3 as primary Ab against Ag 10479 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antigen: Melanoma Antigen Family A, 4
View Anitgen Record

Background

NCI Identification Number:
10479
Antigen Name:
Melanoma Antigen Family A, 4
CPTC Name:
CPTC-MAGEA4
Keywords:
CT1.4; MAGE-41; MAGE-X2; MAGE4; MAGE4A; MAGE4B; MGC21336; OTTHUMP00000025889; OTTHUMP00000025890
Function:
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. The function is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression
Chromosomal Localization:
Xq28
Expression System:
E. Coli
NCBI Accession Number:
BC017723.1
Swiss Prot Accession Number:
P43358
DNA Source:
HIP: HsCD00043768
Vector Name:
pMCSG7
Extinction Coefficient:
38515
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAG
PPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKY
RAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDG
LLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQ
ENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV
Native Sequence:
MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQ
SPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAK
ELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLG
NNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENY
LEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV
Calculated Isoelectric Point:
4.68
Molecular Weight:
35201
Last Updated:
03/19/2009

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-MAGEA4

PAGE of Ag 10479 (with molecular weight standards in lane 1)

Characterization SOP Files

SOPs

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