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CPTC Reagents Data Portal

Fascin

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Antibodies
Antigen
Antibody: CPTC-Fascin-1
View Antibody Record

Background

Catalog Number:
CPTC-Fascin-1
Target Antigen:
Fascin
Isotype:
IgG2a
Species:
Mouse
Last Updated:
08/27/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antibody: CPTC-Fascin-2
View Antibody Record

Background

Catalog Number:
CPTC-Fascin-2
Target Antigen:
Fascin
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/21/2010

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-Fascin-3
View Antibody Record

Background

Catalog Number:
CPTC-Fascin-3
Target Antigen:
Fascin
Isotype:
IgG2b
Species:
Mouse
Last Updated:
08/28/2009

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antigen: Fascin
View Anitgen Record

Background

NCI Identification Number:
10411
Antigen Name:
Fascin
CPTC Name:
CPTC-Fascin
Keywords:
FSCN1; FAN1; FLJ38511; Fascin; HSN; SNL; p55
Function:
Chromosomal Localization:
7p22
Expression System:
E. Coli
NCBI Accession Number:
BC007948
Swiss Prot Accession Number:
Q16658
DNA Source:
HIP:HsCD00004052
Vector Name:
pMCSG7
Extinction Coefficient:
68153
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPD
EAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHR
RYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAV
DRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKV
AFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQ
GMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNAS
CYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFR
GEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSG
DTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Native Sequence:
MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEA
GSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRR
YFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAV
DRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGK
VAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVST
RQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSK
NASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPI
IVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSA
VTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Calculated Isoelectric Point:
6.83
Molecular Weight:
54802
Last Updated:
02/09/2009

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Ag 10411

PAGE of Ag 10411 (with molecular weight standards in lane 1)

Characterization SOP Files

SOPs

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