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CPTC Reagents Data Portal

Calcyclin (Prolactin Receptor Associated Protein)

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Antibodies
Antigen
Antibody: CPTC-Calcyclin-1
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Background

Catalog Number:
CPTC-Calcyclin-1
Target Antigen:
Calcyclin (Prolactin Receptor Associated Protein)
Isotype:
IgG1
Species:
Mouse
Last Updated:
01/21/2010

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Calcyclin-1 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-Calcyclin-1 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-Calcylin is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-Calcylin is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-Calcyclin-2
View Antibody Record

Background

Catalog Number:
CPTC-Calcyclin-2
Target Antigen:
Calcyclin (Prolactin Receptor Associated Protein)
Isotype:
IgG1
Species:
Mouse
Last Updated:
12/22/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-Calcyclin-2 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-Calcyclin-2 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

IHC NCI60

Download 626A13.1.xls (16.0 KB)

Immuno-histochemistry of CPTC-S100A4-3 for NCI60 Cell Line Array at titer 1:250 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG

Characterization SOP Files

NCI 60 Protien Array

Click to enlarge image Protein Array in which CPTC-Calcyclin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Protein Array in which CPTC-Calcyclin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antigen: Calcyclin (Prolactin Receptor Associated Protein)
View Anitgen Record

Background

NCI Identification Number:
10268
Antigen Name:
Calcyclin (Prolactin Receptor Associated Protein)
CPTC Name:
CPTC-Calcyclin
Keywords:
Calcylin, S100A6; S100 calcium binding protein A6; 2A9; 5B10; CABP; CACY; OTTHUMP00000015472; OTTHUMP00000015473; PRA
Function:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
Chromosomal Localization:
1q21
Expression System:
E. Coli
NCBI Accession Number:
BC001431
Swiss Prot Accession Number:
P06703
DNA Source:
HIP:HsCD00002783
Vector Name:
pMCSG7
Extinction Coefficient:
4470
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMACPLDQAIGLLVAIF
HKYSGREGDKHTLSKKELK
ELIQKELTIGSKLQDAEIA
RLMEDLDRNKDQEVNFQEY
VTFLGALALIYNEALKG
Native Sequence:
MACPLDQAIGLLVAIFHK
YSGREGDKHTLSKKELKE
LIQKELTIGSKLQDAEIA
RLMEDLDRNKDQEVNFQE
YVTFLGALALIYNEALKG
Calculated Isoelectric Point:
5.32
Molecular Weight:
10452
Last Updated:
02/09/2009

Links

Characterization Data

Gel

Click to enlarge image Protein Array in which CPTC-Calcyclin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

Ag 10268

PAGE of Ag 10268 (with molecular weight standards in lane 1)

Characterization SOP Files

SOPs

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