Chloride Intracellular Channel 1
Background
Purchase
- Purchase CPTC-CLIC1-1 (Chloride Intracellular Channel 1 - 1) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
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Western Blot using CPTC-CLIC1-1 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
IHC NCI60
Download 639A2.1.xls (16.0 KB)Immuno-histochemistry of CPTC-CLIC1-1 for NCI60 Cell Line Array at titer 1:250 0=NEGATIVE 1=WEAK 2=MODERATE 3=STRONG
Characterization SOP Files
NCI 60 Protien Array
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Protein Array in which CPTC-CLIC1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
Purchase
- Purchase CPTC-CLIC1-2 (Chloride Intracellular Channel 1 - 2) - DSHB
Characterization Data [Compare Characterization Data]
Western Blot
Click to enlarge
Western Blot using CPTC-CLIC1-2 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
Click to enlarge
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protien Array
Click to enlarge
Protein Array in which CPTC-CLIC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
including stabilization of cell membrane potential,transepithelial transport, maintenance of
intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
DTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPK
LAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYL
TSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKK
YRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK
RRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAA
LNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSP
LPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRG
FTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK
Links
- Uniprot - Uniprot CLIC1
- Entrez - Entrez CLIC1
Characterization Data
Gel
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Ag 10458
PAGE of Ag 10458 with molecular weight standards
Characterization SOP Files
SOPs
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