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CPTC Reagents Data Portal

BCL2-like 2

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Antibodies
Antigen
Antibody: CPTC-BCL2L2-1
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Background

Catalog Number:
CPTC-BCL2L2-1
Target Antigen:
BCL2-like 2
Isotype:
IgG1
Species:
Mouse
Last Updated:
08/25/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-BCL2L2-1 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-BCL2L2-1 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antibody: CPTC-BCL2L2-2
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Background

Catalog Number:
CPTC-BCL2L2-2
Target Antigen:
BCL2-like 2
Isotype:
IgG1
Species:
Mouse
Last Updated:
08/27/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-BCL2L2-2 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-BCL2L2-2 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antibody: CPTC-BCL2L2-3
View Antibody Record

Background

Catalog Number:
CPTC-BCL2L2-3
Target Antigen:
BCL2-like 2
Isotype:
IgG2a
Species:
Mouse
Last Updated:
08/27/2009

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-BCL2L2-3 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

Western Blot using CPTC-BCL2L2-3 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

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Antigen: BCL2-like 2
View Anitgen Record

Background

NCI Identification Number:
10179
Antigen Name:
BCL2-like 2
CPTC Name:
CPTC-BCL2L2
Keywords:
BCL2L2; BCL-W; BCLW; KIAA0271
Function:
This gene encodes a pro-survival (anti-apoptotic) member of the bcl-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.

Relative to its Bcl-2 counterparts there is considerably less data on this particular protein however much effort is being made to further understand its cellular roles. Located on chromosome 14q11 it appears to be redundant in most tissues apart from specific examples. It was originally discovered by Gibson et al.

Gibson L, Holmgreen SP, Huang DC, et al. (1996). "bcl-w, a novel member of the bcl-2 family, promotes cell survival.". Oncogene 13 (4): 665
Chromosomal Localization:
14q11.2 - q12
Expression System:
E. Coli
NCBI Accession Number:
NM_004050
Swiss Prot Accession Number:
Q92843
DNA Source:
HIP:HsCD00000436
Vector Name:
pMCSG7
Extinction Coefficient:
33523
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMATPASAPDTRALVADFVGYKLRQKGYVCGAG
PGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQ
LHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFFVF
GAALCAESVNKEMEPLVGQVQEWMVAYLETRLADW
IHSSGGWAEFTALYGDGALEEARRLREGNWA
Native Sequence:
MATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAA
DPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQR
FTQVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEP
LVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGAL
EEARRLREGNWASVRTVLTGAVALGALVTVGAFFASK
Calculated Isoelectric Point:
5.06
Molecular Weight:
18629
Last Updated:
03/03/2009

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

Ag 10179

PAGE of Ag 10179 (with molecular weight standards in lane 1)

Characterization SOP Files

SOPs

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